Twining is a surname. Notable people with the surname include:
Dick Twining (1889–1979), English cricketer
Edward Twining (1899–1967), British diplomat and author
Elizabeth Twining (1805–1889), English botanical illustrator
Ernest W. Twining (1875–1956), English modelmaker, artist, and engineer
James Twining (born 1972), British author of thriller novels
Louisa Twining (1820–1912), social reformer
Luella Twining (1871–1939), Labor activist
Mary Twining (1726-1804), tea merchant, mother of Richard Twining
Merrill B. Twining (1902–1996), American Marine Corps general
Nathan Crook Twining (1869–1924), American Navy admiral
Nathan Farragut Twining (1897–1982), American Air Force general, Air Force Chief of Staff; Chairman Joint Chiefs of Staff 1957–1960
Richard Twining (1749–1824), tea merchant
Thomas Twining (merchant) (1675–1741), English merchant and founder of the Twinings tea company
Thomas Twining (scholar) (1735–1804), English classical scholar
Twink Twining (1894–1973), American baseball player
William Twining (military physician) (1790–1835), British military physician
William Twining (born 1934), English professor of law
Charles H. Twining (born 1940), career US Diplomat