Theater roles
In 2000, Bierko made his Broadway debut as Professor Harold Hill in Susan Stroman's critically acclaimed revival of Meredith Willson's The Music Man, for which he received a Tony Award nomination.
Bierko was slated to appear on Broadway in the Manhattan Theatre Club production of To Be or Not to Be but withdrew from the production August 29, 2008, for creative differences. He starred as Sky Masterson in the Broadway revival of Guys and Dolls which began performances at the Nederlander Theatre on February 5, 2009, and officially opened on March 1, 2009. It closed on June 14, 2009, after 113 performances. He performed the role of Peter in the acclaimed 2011 New York Philharmonic production of Stephen Sondheim's Company with Neil Patrick Harris, Patti Lupone and Stephen Colbert.
Bierko replaced Bertie Carvel as Miss Trunchbull in Matilda the Musical at the Shubert Theatre along with Jill Paice as Miss Honey on September 3, 2013, but could not start performances until September 17 following a neck and shoulder injury he suffered rehearsing a gymnastic flip. Despite the injury, Bierko continued rehearsing, eventually performing the role for about a month before the shoulder injury – along with a foot injury sustained during performance – made it impractical to continue.
In 2022, Conor McPherson cast Bierko as Mr. Burke in his Girl from the North Country (featuring music by Bob Dylan) opposite Luba Mason and Mare Winningham. The Tony Award-winning limited engagement played its final performance June 19, 2022.
