Recurring
Lucy the Dragonfly is a dragonfly with kaleidoscope eyes who is a sleep doctor.
Boris the Frog (Doron Bell, singing Aloe Blacc) is a frog who loves things Mother Nature provided.
Postman Bee (Brian Drummond; singing Wesley Schultz) is a bee who, as his name implies, works as a postal worker. He is never late and always gets his deliveries right, unless he is feeling sad about something. He cares a great deal for his grandmother, Granny Bee (Nicole Oliver) who was once a famous TV chef known for her honey pie.
Doris the Spider (Gwynyth Walsh) is an elderly arachnid who is always filling her garden with beautiful art pieces that are made from her webbing and various other objects found in the backyard. She teaches the bugs to see the beauty in everything.
Geoff the Cockroach (Peter Kelamis; singing Dave Faulkner) is an opportunistic roach who acts like a sleazy business man. He typically sells homes to the residents in the backyard, implying that he is a realtor. He gets along with Alex the Stinkbug.
Alex the Stinkbug (Fred Ewanuick) is a jokey bug that is always farting. His farts are powerful enough to power machines built by Crick. He can be uncouth, but is very friendly. He gets along with Geoff the Cockroach.
Dr. Robert (Ian Hanlin) is a mantis in the medical profession. He is very friendly and has perfect bedside manners. He always seems to know the perfect treatment for certain ailments.
Katter (Britt McKillip; singing Frances) is a pretty caterpillar who later transforms into a butterfly. She is soft-spoken and shy, but is very friendly with everyone. She is best friends with Morgs and Millie.
Morgs (David Paul Grove; singing James Corden) is a very nervous and self-conscious stick bug. He has trouble standing up for himself and wishes that he wasn't "just a stick". He is best friends with Katter and Millie.
Millie Pede (Maryke Hendrikse; singing Tori Kelly) is a multifooted millipede who is kind and reserved. She is unsure of her dancing ability, but with help from her friends learns to do so effectively. She is best friends with Morgs and Katter.
Mr. Sun (Michael Caton; singing Robbie Williams and Yusuf Islam / Cat Stevens) is the large all seeing star during the daytime. Despite his largeness, he can speak directly to the bugs and has even sent and received letters from them. In All Together Now, Mr. Sun now has a beard and arms.
Mr. Moonlight (Robert Alexander and Charles Demers) is Mr. Sun's nighttime counterpart. He can be playful with the bugs, but sometimes feels that they like Mr. Sun more than him.
Dr. Robot (Peter Kelamis; singing Ev Jones) is a special robot that Crick built to help his friends while Dr. Robert was on vacation. Despite a few kinks here and there he has proven to be efficient. When Dr. Robert returned, Dr. Robot became Crick's assistant for his experiments.
Blackbird (Bethany Brown; singing Sia, Regina Spektor, and Jennifer Hudson) is a bird that always has her head in the clouds, both figuratively and literally. The bugs helped her damaged wing and in return, she stuck around to help them in any way she could.
Mr. Mudwasp (Andrew Hansen; singing Daniel Johns), a wasp, and working man who lives in a hive that he is constantly renovating. He is always trying to speak in foreign terms. He has numerous wasp children with his constantly pregnant wife, Mrs. Mudwasp (Ashleigh Ball). They were once identified as the Hendersons. Mr. Mudwasp has also been identified as Baz.
The Ants (Kyle Rideout) are the colony workers of the backyard. They all think uniformly as one and thus have a hard time acting as separate people. They all live in one ant hill and become lost if anything happened to it.
Freda (Shannon Chan-Kent; singing Kate Miller-Heidke) is Walter's long lost sister introduced in All Together Now. She is a very ditsy slug that shares many similarities and differences with her twin brother. She moves into a house next door to Walter. She is named after the Beatles' secretary Freda Kelly.
Glowies are glow bugs that act as a full light source for all the bugs. They always seem to be angry or annoyed at everyone, possibly because they are constantly used all the time and are treated more like objects. Nevertheless, they do their job effectively.
Octopus (Jay Brazeau) is a talking sprinkler toy that resembles a purple octopus. He only communicates with the bugs and provides comfort to everyone by spraying water. However, he is only capable of doing so when the hose he is connected to is on.
Bulldog (Colin Murdock; singing James Bay) is a big friendly slobbering dog. He initially had no home or way to communicate with the bugs, but Crick builds a translator and is adopted by the humans.
Mee-Yow (Ashleigh Ball) is a cat that is owned by the humans. He roams about the yard and, while not necessarily mean, is shown to be rather dangerous to the bugs as he tends to attack and play with them.
The Man (JR Bourne and Ian Hanlin) and Julia's Mom (Erin Mathews) are Gigants, they are what the bugs refer to humans whom the bugs are fascinated by. They live in the "Big House" at the edge of the backyard. They have a daughter named Julia, and have a second daughter named Jude in All Together Now.
Julia (Rebecca Husain) is the first born daughter of the humans who live in the "Big House". Jay seems to relate to her. As of All Together Now, Julia becomes a big sister after her mom has a baby girl named Jude.
